MSH6 Antibody

• Description:

  MSH6 is a component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P52701

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 374-540 from the human protein were used as the immunogen for this MSH6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MTS6-1

• Applications:

  WB, IHC-P, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MSH6 antibody is available for research use only.

• Storage Conditions:

  Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of human HeLa lysate with MSH6 antibody (clone MTS6-1) . Expected molecular weight: 120-160 kDa depending on phosphorylation level.