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Recombinant CD15 Antibody

CAT: 0800-V7257SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7257SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
CAS Number
9000-83-3
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P22083
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
FUT4/1478R
Type
Recombinant
Applications
FACS, IF, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant CD15 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD15 antibody is available for research use only.
Storage Conditions
Store the recombinant CD15 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic and cell surface
Image Legend
Immunofluorescent staining of human U937 cells with recombinant CD15 antibody (clone FUT4/1478R, green) and Reddot nuclear stain (red) .

UniProtKB · P22083

Alpha-(1,3)-fucosyltransferase 4

FUT4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P22083
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FUT4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2RMS0
Protein keywords

Coding sequence diversity

Alternative initiation

PTM

Glycoprotein

Molecular function

GlycosyltransferaseTransferase

Cellular component

Golgi apparatusMembrane

Biological process

Inflammatory response

Technical term

Proteomics identificationReference proteome

Domain

Signal-anchorTransmembraneTransmembrane helix

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