Recombinant TNF-alpha Antibody / Rabbit Monoclonal

• Description:

  The primary role of TNF is in the regulation of immune cells. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD) . [Wiki]

• Specifications:

  Immunohistochemistry (FFPE) : 2-5 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P01375

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for the recombinant TNF-alpha antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  RMTNFA-1

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the recombinant TNF-alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant TNF-alpha antibody is available for research use only.

• Storage Conditions:

  Store the recombinant TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the recombinant TNF-alpha antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic and extracellular (secreted)

• Image Legend:

  IHC staining of FFPE human histiocytoma with recombinant TNF alpha antibody (clone RMTNFA-1) . Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.