Nucleolin Antibody

• Description:

  Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P19338

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant human protein was used as the immunogen for the Nucleolin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  NPC23-2

• Applications:

  FACS, IHC-P, WB, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Nucleolin antibody is available for research use only.

• Storage Conditions:

  Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Nucleoli

• Image Legend:

  IHC testing of FFPE human tonsil with Nucleolin antibody (clone NPC23-2) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.