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NCL Antibody / Nucleolin

CAT: 0800-RQ8805Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-RQ8805Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. [UniProt]
Specifications
Western blot: 1:500-1:2000
UniProt
P19338
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human Nucleolin protein was used as the immunogen for the NCL antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
29N05
Applications
WB
Purity
Affinity chromatography
Format
Purified
Limitations
This NCL antibody is available for research use only.
Storage Conditions
Store the NCL antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the NCL antibody should be determined by the researcher.
Image Legend
Western blot testing of human HeLa cell lysate with NCL antibody. Predicted molecular weight ~77 kDa, commonly observed at 100~110 kDa.

UniProtKB · P19338

Nucleolin

NUCL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P19338
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NCL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q53SK1, Q8NB06, Q9UCF0, Q9UDG1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationPhosphoproteinUbl conjugation

Cellular component

CytoplasmNucleus

Molecular function

DNA-bindingRNA-binding

Domain

Repeat

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