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TGF alpha Antibody

CAT: 0800-V7158IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7158IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transforming growth factor alpha is a protein that in humans is encoded by the TGFA gene. As a member of the epidermal growth factor (EGF) family, TGFa is a mitogenic polypeptide. The protein becomes activated when binding to receptors capable of protein kinase activity for cellular signaling. TGFa is a transforming growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. [Wiki]
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P01135
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length TGF protein was used as the immunogen for the TGF alpha antibody. (3)
Clonality
Monoclonal
Isotype
IgG1 k
Clone
TFGFa-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the TGF alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TGF alpha antibody is available for research use only.
Storage Conditions
Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic and extracellular (secreted)
Image Legend
IHC testing of FFPE human testicular carcinoma with TGF alpha antibody (clone TFGFa-1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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