SUMO2/3 Antibody

CAT: 0800-V7091-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V7091-20UGSize:20 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer�s disease.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human SUMO2 protein was used as the immunogen for this SUMO2/3 antibody. This antibody reacts with both SUMO2 and SUMO3.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
S23MT-1
Applications
WB, IHC-P, IF
Purity
Protein G purified
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the SUMO2/3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This SUMO2/3 antibody is available for research use only.
Storage Conditions
Store the SUMO2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the SUMO2/3 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Predominantly nuclear with some cytoplasmic staining
Image Legend
Western blot testing of human HeLa lysate with SUMO2/3 antibody (clone S23MT-1) .
Product Datasheet
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