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P21 Antibody

CAT: 0800-V7072SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7072SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
P21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma) .
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P38936
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the p21 antibody. This antibody is not suitable for testing mouse and rat samples.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
WAF21-1
Applications
IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the p21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p21 antibody is available for research use only.
Storage Conditions
Store the p21 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Nuclear
Image Legend
IHC testing of human bladder carcinoma and p21 antibody (WAF21-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
Product Datasheet
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