Products for Research Use Only

TAG-72 Antibody

CAT: 0800-V7058-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7058-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1)
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human TAG-72 protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
TAGP-4
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the TAG-72 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This TAG-72 antibody is available for research use only.
Storage Conditions
Store the TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic and cell surface
Image Legend
IHC testing of human ovarian carcinoma with TAG-72 antibody (clone TAGP-4) .