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V5 Tag Antibody

CAT: 0800-F52064-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F52064-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5) . NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
Host
Rabbit
Immunogen
The V5 sequence GKPIPNPLLGLDST was used as the immunogen for the V5 tag antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the V5 Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This V5 Tag antibody is available for research use only.
Storage Conditions
Aliquot the V5 Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the V5 Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Lanes: 1: non-transfected cells; 2: V5-tagged empty plasmid; and V5-tagged proteins: 3-A, 4-B, 5-C, 6-D; Data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.

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