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CD8a Antibody

CAT: 0800-V7034-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7034-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.
CAS Number
9007-83-4
Specifications
Immunohistology (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for this CD8a antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CLDA8a-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
CD8a antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD8a antibody is available for research use only.
Storage Conditions
CD8a antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cell surface
Image Legend
IHC analysis of FFPE human tonsil and CD8a antibody (clone CLDA8a-1) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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