Vimentin Antibody

• Description :

  Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt :

  P08670

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Full length human recombinant protein was used as the immunogen for the Vimentin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  VIM66-1

• Applications :

  WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Vimentin antibody is available for research use only.

• Storage Conditions :

  Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  Western blot testing of Vimentin antibody (clone VIM66-1) and Raji cell lysate. Predicted molecular weight ~58 kDa.