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Ki67 Antibody

CAT: 0800-V3871-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3871-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P46013
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
PMKI67-1
Applications
IHC-P, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Ki67 antibody is available for research use only.
Storage Conditions
Store the Ki67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear
Image Legend
IHC testing of FFPE human tonsil stained with Ki67 antibody (clone PMKI67-1) . Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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