Ki67 Antibody

• Description:

  Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

• UniProt:

  P46013

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MKI67/2462

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Ki67 antibody is available for research use only.

• Storage Conditions:

  Store the Ki67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Ki67 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC testing of FFPE human skin stained with Ki67 antibody (MKI67/2462) . Required HIER: boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.