CD103 Antibody / Integrin alpha E

CAT: 0800-V3837SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3837SAF-100UGSize:100 µg
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Description
This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P38570
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ITGAE/2474
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD103 antibody is available for research use only.
Storage Conditions
Store the CD103 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Titering of the CD103 antibody may be required for optimal performance.
Location
Cell membrane, cytoplasm
Image Legend
IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2474) . HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.

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