CD103 Antibody / Integrin alpha E

• Description :

  This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL) .

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P38570

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  ITGAE/2474

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD103 antibody is available for research use only.

• Storage Conditions :

  Store the CD103 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Titering of the CD103 antibody may be required for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell membrane, cytoplasm

• Image Legend :

  IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2474) . HIER: boil tissue sections in pH9, 1mM EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.