Mesothelin Antibody / MSLN

• Description:

  Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

• Specifications:

  Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

• UniProt:

  Q13421

• Host:

  Mouse

• Reactivity:

  Human, Mouse and Rat

• Immunogen:

  A portion of amino acids 273-407 from the human protein was used as the immunogen for the Mesothelin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MSLN/2131

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Biotin Conjugate

• Buffer:

  0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Mesothelin antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

• Limitations:

  This Mesothelin antibody is available for research use only.

• Storage Conditions:

  Store the Mesothelin antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

• Formulation:

  0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Mesothelin antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell surface, secreted

• Image Legend:

  IHC staining of FFPE human endometrial carcinoma with biotinylated Mesothelin antibody (clone MSLN/2131) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.