CD40 Antibody

• Description:

  This antibody is specific for the soluble form of CD40. It is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells

• UniProt:

  P25942

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  C-terminal region amino acids RDPVCHPLGA were used as the immunogen for the CD40 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  T8P2G4*A6

• Applications:

  IF, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD40 antibody is available for research use only.

• Storage Conditions:

  Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD40 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD40 antibody (clone T8P2G4*A6) as confirmation of integrity and purity.