Recombinant CD45 Antibody / Rabbit Monoclonal

• Description:

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT (1)

• UniProt:

  P08575

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as immunogen for this recombinant CD45 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  CDLA45-3R

• Type:

  Recombinant

• Applications:

  IHC-P, FACS

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  The recombinant CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This recombinant CD45 antibody is available for research use only.

• Storage Conditions:

  The recombinant CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9000-83-3

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  IHC staining of FFPE human tonsil tissue with recombinant CD45 antibody (clone CDLA45-3R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.