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Recombinant CD43 Antibody

CAT: 0800-V3652-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3652-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
CAS Number
9000-83-3
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16150
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the recombinant CD43 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RSPN/1094
Type
Recombinant
Applications
WB, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD43 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD43 antibody is available for research use only.
Storage Conditions
Store the recombinant CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
IHC testing of FFPE human tonsil with recombinant CD43 antibody (clone rSPN/1094) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

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