CD43 Antibody

• Description:

  It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P16150

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human SPN protein was used as the immunogen for the CD43 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  MT-1

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the CD43 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD43 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD43 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD43 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD43 antibody (clone MT-1) . Inset: PBS instead of primary antibody, secondary negative control. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.