Recombinant Villin Antibody / VIL1

• Description:

  Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P09327

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 179-311 from the human protein were used as the immunogen for this recombinant Villin antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  RVIL1/1325

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the recombinant Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Villin antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  IHC testing of FFPE human small intestine carcinoma with recombinant Villin antibody (clone rVIL1/1325) . Required HIER: boil tissue sections in pH9 EDTA buffer, for 10-20 min followed by cooling at RT for 20 min.