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Recombinant CD27 Antibody

CAT: 0800-V3558-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3558-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P26842
Host
Mouse
Reactivity
Human
Immunogen
Human CD27 protein was used as the immunogen for the recombinant CD27 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RLPFS2/1611
Type
Recombinant
Applications
FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD27 antibody is available for research use only.
Storage Conditions
Store the recombinant CD27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell surface
Image Legend
IHC testing of FFPE human tonsil tissue with recombinant CD27 antibody (clone rLPFS2/1611) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.

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