S100B Antibody

• Description:

  S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. [Wiki]

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Immunohistology (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P04271

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PS1B1-1

• Applications:

  IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This S100B antibody is available for research use only.

• Storage Conditions:

  Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green) . F-actin filaments were labeled with DyLight 554 Phalloidin (red) . DAPI was used to stain the cell nuclei (blue) .