Elastase 3B Antibody / CELA3B

• Description :

  Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

• Specifications :

  ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P08861

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CELA3B/1257

• Applications :

  ELISA, WB, IHC-P

• Purity :

  Protein G

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Elastase 3B antibody is available for research use only.

• Storage Conditions :

  Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the Elastase 3B antibody should be determined by the researcher.1. FFPE staining requires

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.