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S100B Antibody

CAT: 0800-V3419-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V3419-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0) . This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunfluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P04271
Host
Mouse
Reactivity
Human
Immunogen
Purified bovine brain S100 protein was used as the immunogen for this S100B antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
SPM354
Applications
IHC-P, WB, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This S100B antibody is available for research use only.
Storage Conditions
Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the S100B antibody for each application should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
IHC testing of FFPE human melanoma with S100B antibody (clone SPM354) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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