CDX2 Antibody

CAT: 0800-V3415-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3415-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q99626
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 150-249 from the human protein were used as the immunogen for this CDX2 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CDX2/1690
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CDX2 antibody is available for research use only.
Storage Conditions
Store the CDX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDX2 antibody to be titered up or down for optimal performance.
Location
Nuclear
Image Legend
IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690) . Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.

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