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PAX8 Antibody

CAT: 0800-V3413-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3413-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PAX8 (and PAX2) is one of the important regulators of urogenital system morphogenesis. They play a role in the specification of the first renal cells of the embryo and remain essential players throughout development. [Wiki]
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q06710
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PAX8/1492
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the PAX8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PAX8 antibody is available for research use only.
Storage Conditions
Store the PAX8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Nuclear, cytoplasmic
Image Legend
IHC testing of FFPE human thyroid carcinoma with PAX8 antibody (clone PAX8/1492) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
Product Datasheet
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