FOXA1 Antibody

• Description :

  The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (α and β), HNF-3 (α, β and γ), HNF-4 (α and γ), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER) -positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 2-4 µg/mL

• UniProt :

  P55317

• Host :

  Mouse

• Reactivity :

  Human, Rat

• Immunogen :

  A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  FOXA1/1518

• Applications :

  WB, IHC-P, FACS, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This FOXA1 antibody is available for research use only.

• Storage Conditions :

  Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

• Location :

  Nuclear

• Image Legend :

  Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FOXA1/1518) . Predicted molecular weight: ~49 kDa.