MCM7 Antibody

• Description:

  Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P33993

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 195-319 were used as the immunogen for the MCM7 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MCM7/1466

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MCM7 antibody is available for research use only.

• Storage Conditions:

  Store the MCM7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of human samples with MCM7 antibody (clone MCM7/1466) . Expected molecular weight: 80-90 kDa.