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MCM4 Antibody

CAT: 0800-RQ6084Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6084Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Minichromosome maintenance, S. cerevisiae, homolog of, 4, also called CDC21, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The 864-amino acid protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial. The gene encodes a subunit of the MCM2-7 complex (also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.
CAS Number
9007-83-4
Specifications
Western blot: 0.25-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P33991
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human partial protein (amino acids 361-677) was used as the immunogen for this MCM4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the MCM4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MCM4 antibody is available for research use only.
Storage Conditions
After reconstitution, the MCM4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MCM4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Immunofluorescent staining of FFPE human A431 cells with MCM4 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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