PSAP Antibody

• Description:

  Prostate specific acid phosphatase (PSAP) is an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P15309

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  PSAP purified from human seminal plasma was used as the immunogen for this PSAP antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM312

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  The PSAP antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This PSAP antibody is available for research use only.

• Storage Conditions:

  The PSAP antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the PSAP antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC testing of human prostate carcinoma with PSAP antibody (clone SPM312) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.