CD3e Antibody

• Description:

  The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.

• Specifications:

  Western blot: 1-2 µg/mL for 60 min at RT, Flow cytometry: 1-2ug/10^6 cells

• UniProt:

  P07766

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  C3e/1308

• Applications:

  WB, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD3e antibody is available for research use only.

• Storage Conditions:

  Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD3e antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  Western blot testing of human Jurkat cell lysate with CD3e antibody (clone C3e/1308) . Predicted molecular weight ~23 kDa.