MMP2 Antibody

• Description:

  It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP2 secretion requires the Ras signaling pathway.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P08253

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 195-319 were used as the immunogen for the MMP2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  MMP2/1501

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the MMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MMP2 antibody is available for research use only.

• Storage Conditions:

  Store the MMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell surface

• Image Legend:

  Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501) . Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form) .