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MMP2 Antibody

CAT: 0800-V3247SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3247SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP2 secretion requires the Ras signaling pathway.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P08253
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 195-319 were used as the immunogen for the MMP2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MMP2/1501
Applications
WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the MMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MMP2 antibody is available for research use only.
Storage Conditions
Store the MMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, cell surface
Image Legend
Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501) . Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form) .

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