BRCA1 Antibody

• Description :

  BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC) . This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

• CAS Number :

  9007-83-4

• Specifications :

  Immunohistochemistry (FFPE) : 2-5 µg/mL for 60 min at RT

• UniProt :

  P38398

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  BRCA1/1398

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the BRCA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This BRCA1 antibody is available for research use only.

• Storage Conditions :

  Store the BRCA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the BRCA1 antibody should be determined by the researcher.

• Location :

  Nuclear, cytoplasmic

• Image Legend :

  IHC testing of FFPE human breast carcinoma tissue with BRCA1 antibody (clone BRCA1/1398) . HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.