CD15 Antibody

• Description:

  CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

• Specifications:

  Flow cytometry: 2-4ug/10^6 cells, Immunofluorescence: 2-4 µg/mL, Immunohistochemistry (FFPE) : 5-10 µg/mL for 30 min at RT

• UniProt:

  P22083

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Purified human neutrophils were used as the immunogen for the CD15 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein A affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD15 antibody is available for research use only.

• Storage Conditions:

  Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD15 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.