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Calnexin Antibody

CAT: 0800-V3199-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3199-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER) -associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL
UniProt
P27824
Host
Mouse
Reactivity
Human
Immunogen
A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
CANX/1541
Applications
ELISA, WB, IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Calnexin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Calnexin antibody is available for research use only.
Storage Conditions
Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the Calnexin antibody may be required for optimal performance.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.

UniProtKB · P27824

Calnexin

CALX_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P27824
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CANX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2R5V8, B4DGP8, B4E2T8, D3DWQ3, D6R9K3
Protein keywords

PTM

AcetylationDisulfide bondLipoproteinPalmitatePhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

CalciumLectinMetal-binding

Molecular function

Chaperone

Technical term

Direct protein sequencingProteomics identificationReference proteome

Cellular component

Endoplasmic reticulumMembraneMitochondrion

Biological process

Host-virus interaction

Domain

RepeatSignalTransmembraneTransmembrane helix
Product Datasheet
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