CD53 Antibody

• Description :

  Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1) . CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

• Specifications :

  Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt :

  P19397

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Human Sezary cells were used as the immunogen for the CD53 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  63-5A3

• Applications :

  Functional studies, FACS, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD53 antibody is available for research use only.

• Storage Conditions :

  Store the CD53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD53 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  SDS-PAGE Analysis of Purified, BSA-Free CD53 Antibody (clone 63-5A3) . Confirmation of Integrity and Purity of the Antibody.