PECAM Antibody

• Description:

  CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 mAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1:50-1:100 for 30 min at RT

• UniProt:

  P16284

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of CD31/PECAM was used as the immunogen for the PECAM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  1A10

• Applications:

  WB, IHC-P

• Purity:

  Unpurified high titer supernatant

• Format:

  Bioreactor concentrate

• Buffer:

  Bioreactor concentrate with 0.05% sodium azide

• Reconstitution:

  Store the PECAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PECAM antibody is available for research use only.

• Storage Conditions:

  Store the PECAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Bioreactor concentrate with 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasm of endothelial cells

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human tonsil stained with PECAM antibody (1A10)