NF-H Antibody (phospho)

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CAT#:0800-V2759SAF-100UGSize:100 µg

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Description

This mAb reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H) . It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H) . Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P12036

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho) .

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NE14

Applications

WB, FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the NF-H antibody (phospho) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NF-H antibody (phospho) is available for research use only.

Storage Conditions

Store the NF-H antibody (phospho) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with NF-H antibody (NE14) .

UniProtKB · P12036

Neurofilament heavy polypeptide

NFH_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P12036
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: NEFH
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: NF-H
EC number: —
Processing: —
Secondary accessions: B4DYY4, Q96HF8, Q9UJS7, Q9UQ14

Protein keywords

Coding sequence diversity

Alternative splicing

Disease

Amyotrophic lateral sclerosisCharcot-Marie-Tooth diseaseDisease variantNeurodegenerationNeuropathy

Cellular component

Cell projectionCytoplasmCytoskeletonIntermediate filament

Domain

Coiled coilRepeat

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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Alternative Products

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GTR18081450-01	NF-H Antibody (phospho)
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APR11336G	Monoclonal Neurofilament, phospho (NF-H) (Neuronal Marker) Antibody, Clone: NE14
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