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EpCAM Antibody

CAT: 0800-V2689CF488-100TSize: 100 TestsDry Ice: NoHazardous: No
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CAT#:0800-V2689CF488-100TSize:100 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Small cell lung carcinoma cells were used as the immunogen.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM134
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the EpCAM antibody at 2-8oC, protected from light.
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Store the EpCAM antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Location
Cell surface
Image Legend
Flow cytometry testing of PFA-fixed human MCF7 cells with unlabeled anti-EpCAM antibody (clone SPM134) + CF488-labeled secondary; Red=isotype control, Blue= anti-EpCAM antibody.
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