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ICAM-3 Antibody

CAT: 0800-V2607-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2607-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P32942
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
101-1D2
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the ICAM-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ICAM-3 antibody is available for research use only.
Storage Conditions
Store the ICAM-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Location
Membrane, cytoplasm
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free ICAM-3 Antibody (clone 101-1D2) . Confirmation of Integrity and Purity of the Antibody.

UniProtKB · P32942

Intercellular adhesion molecule 3

ICAM3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P32942
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ICAM3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ICAM-3
EC number
—
Processing
Precursor
Secondary accessions
Q6PD68
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesionPhagocytosis

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix
Product Datasheet
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