HLA-A Antibody

HLA-A Antibody

• Description: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This mAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and Aw33). Its epitope maps between aa65-to-aa80 of the alpha1 domain of the HLA-A. This mAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
• UniProt: P30443
• Host: Mouse
• Immunogen: Normal human peripheral blood lymphocytes were used as the immunogen for the HLA-A antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: FACS, IF
• Format: Purified
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Reconstitution: Store the HLA-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This HLA-A antibody is available for research use only.