HLA-A25 Antibody (HLA-Aw32)

• Description:

  This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P30443

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32) .

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  SPM417

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the HLA-A25 antibody (HLA-Aw32) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HLA-A25 antibody (HLA-Aw32) is available for research use only.

• Storage Conditions:

  Store the HLA-A25 antibody (HLA-Aw32) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-Aw32 / HLA-A25 antibody (SPM417) .