CD13 Antibody

• Description:

  Recognizes an integral membrane glycoprotein of 150kDa, identified as CD13 (also known as aminopeptidase-N) . The antibody recognizes an extracellular epitope. The CD13 antigen is present on most cells of myeloid origin including granulocytes, monocytes, mast cells, and GM-progenitor cells. It is also expressed by the majority of AML, CML in myeloid blast crisis, and in a smaller fraction of lymphoid leukemias. CD13 is absent from normal lymphocytes, platelets and erythrocytes. CD13 is also present on fibroblasts; endothelial cells, epithelial cells from renal proximal tubules and intestinal brush border, bone marrow stromal cells, osteoclasts, and cells lining bile duct canaliculi. CD13 is identical to aminopeptidase N (APN), a prominent membrane-bound metalloprotease present on the surface of intestinal brush border and renal tubules. CD13 plays a role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal activities. It also serves as a receptor for human coronaviruses (HCV) . The lineage-restricted pattern of expression of CD13 within the hemopoietic compartment suggests that it may be important in myeloid cell differentiation.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL

• UniProt:

  P15144

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human AML cells were used as the immunogen for the CD13 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  WM15

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD13 antibody is available for research use only.

• Storage Conditions:

  Store the CD13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD13 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD13 Antibody (WM15) . Confirmation of Integrity and Purity of the Antibody.