CELA3B Antibody

• Description:

  This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

• Specifications:

  ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 0.5-1 µg/mL

• UniProt:

  P08861

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A partial recombinant protein (aa 82-238) was used as the immunogen for the CELA3B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CELA3B/1218

• Applications:

  ELISA, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CELA3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CELA3B antibody is available for research use only.

• Storage Conditions:

  Store the CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CELA3B antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot of 1) partial recombinant protein, 2) Panc-28 and 3) PANC1 cell lysate using CELA3B antibody (CELA3B/1218) . Predicted molecular weight ~29 kDa.