CD16 Antibody

• Description:

  Recognizes a protein of 50-65 kDa, identified as CD16 (Workshop IV; Code N39) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11) . CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI) -anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

• Specifications:

  Functional studies (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P08637

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  HuNK2

• Applications:

  Functional studies, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD16 antibody is available for research use only.

• Storage Conditions:

  Store the CD16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD16 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD16 Antibody (clone HuNK2) . Confirmation of Integrity and Purity of the Antibody.