CD32 Antibody / FCGR2A

• Description:

  This mAb reacts with a CD32 (FcgRII) epitope distinct from that defined by mAb 8.26 and the epitope overlaps with that of mAb 7.30 (cluster 4) . It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

• Specifications:

  Functional studies (order BSA/sodium azide-free format), Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  P12318

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  8.7

• Applications:

  Functional studies, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD32 antibody is available for research use only.

• Storage Conditions:

  Store the CD32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD32 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, plasma membrane

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free CD32 Antibody (clone 8.7) . Confirmation of Integrity and Purity of the Antibody.