CD20 Antibody

• Description:

  This antibody recognizes a protein of 33-37kDa, identified as CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

• Specifications:

  Flow cytometry: 2-5ul/10^6 cells, Immunofluorescence: 1:100-1:200, Immunohistochemistry (FFPE) : 1:100-1:200 for 30 min at RT

• UniProt:

  P11836

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human tonsil B cells were used as the immunogen for this CD20 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  SPM494

• Applications:

  FACS, IF, IHC-P

• Format:

  Bioreactor concentrate

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD20 antibody is available for research use only.

• Storage Conditions:

  CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  Bioreactor concentrate with 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly cell surface with some cytoplasmic

• Image Legend:

  IHC testing of CD20 antibody and FFPE human lymphoma tissue (clone SPM494) .