CD19 Antibody (B-lymphocyte marker)

• Description:

  CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating)

• UniProt:

  P15391

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human CD19 recombinant protein was used as immunogen for this CD19 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  C19/366

• Applications:

  ELISA

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD19 antibody is available for research use only.

• Storage Conditions:

  CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD19 antibody (clone C19/366) as confirmation of integrity and purity.