CD8a Antibody

• Description :

  CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked heterodimer or homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P01732

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Human CD8 recombinant protein was used as immunogen for this CD8a antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  SPM548

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  CD8a antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations :

  This CD8a antibody is available for research use only.

• Storage Conditions :

  CD8a antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC testing of CD8a antibody (clone SPM548) and FFPE human tonsil tissue.